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RNA polymerase II CTD repeat YSPTSPS (phosphor S2) Recombinant Rat mAb (S-R199)

DNA-directed RNA polymerase II subunit RPB1,RNA polymerase II subunit B1,DNA-directed RNA polymerase II subunit A,DNA-directed RNA polymerase III largest subunit,RNA-directed RNA polymerase II subunit RPB1,POLR2A

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0284

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rat
抗原名称

RNA polymerase II CTD repeat YSPTSPS (phospho S2)
分子别名

DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1; POLR2A
细胞定位

Cytoplasm, Nucleus
Accession

P24928
克隆号

S-R199
抗体类型

Recombinant mAb
抗体同种型

IgG1
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein G
浓度

0.4 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

IHC-P ?

ICC ?

WB

IF ?
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms
IHC 1:400
ICC 1:100 Hu
IF 1:100 Ms, Rt
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms
IHC	1:400
ICC	1:100	Hu
IF	1:100	Ms, Rt
ChIP	1:20-1:50	Hu

背景介绍

RNA polymerase II (RNAP II and Pol II) is a multiprotein complex that transcribes DNA into precursors of messenger RNA (mRNA) and most small nuclear RNA (snRNA) and microRNA. The purified enzyme has typically 10–12 subunits (12 in humans and yeast). The subunit RPB1 contains a carboxy terminal domain (CTD) composed of up to 52 heptapeptide repeats (YSPTSPS) that are essential for polymerase activity.

免疫印迹
- WB result of RNA polymerase II CTD repeat YSPTSPS (phospho S2) Rat mAb
  Primary antibody: RNA polymerase II CTD repeat YSPTSPS (phospho S2) Rat mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: 293T whole cell lysate 20 µg
  Lane 3: HeLa whole cell lysate (phosphatase treated) 20 µg
  Lane 4: 293T whole cell lysate (phosphatase treated) 20 µg
  Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 217 kDa
  Observed MW: 270 kDa
  Exposure time: 90 s
- WB result of RNA polymerase II CTD repeat YSPTSPS (phospho S2) Rat mAb
  Primary antibody: RNA polymerase II CTD repeat YSPTSPS (phospho S2) Rat mAb at 1/1000 dilution
  Lane 1: RAW 264.7 whole cell lysate 20 µg
  Lane 2: C2C12 whole cell lysate 20 µg
  Lane 3: RAW264.7 whole cell lysate (phosphatase treated) 20 µg
  Lane 4: C2C12 whole cell lysate (phosphatase treated) 20 µg
  Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 217 kDa
  Observed MW: 270 kDa
  Exposure time: 90 s
免疫组化
- IHC shows positive staining in paraffin-embedded human testis. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody was used at 1/400 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human testis (phosphatase treated). Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody was used at 1/400 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse testis. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody was used at 1/400 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded mouse testis (phosphatase treated). Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody was used at 1/400 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse stomach. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody was used at 1/400 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat testis. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody was used at 1/400 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded rat testis (phosphatase treated). Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody was used at 1/400 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat stomach. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody was used at 1/400 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells (Right, Green) and negative staining in phosphatase treated (37 °C, 2h) HeLa cells (Left). Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody was used at 1/100 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rat IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.
免疫荧光
- IF shows positive staining in paraffin-embedded mouse testis. Anti- RNA polymerase II CTD repeat YSPTSPS (phosphor S2) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Rat IgG(H+L) (Alexa Fluor® 647 Conjugate) (S0B4049) was used as secondary antibody at 1/2000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded rat testis. Anti- RNA polymerase II CTD repeat YSPTSPS (phosphor S2) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Goat Anti-Rat IgG(H+L) (Alexa Fluor® 647 Conjugate) (S0B4049) was used as secondary antibody at 1/2000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used RNA polymerase II CTD repeat YSPTSPS (phospho S2) Recombinant Rat mAb (S-R199) and beads only at 1:20 for immunoprecipitation.
  Post - immunoprecipitation, both samples
  were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR (%input: immunoprecipitated DNA/input DNA)
  showed the enrichment of RPL30, GAPDH, MYOD1,
  AFM, SAT-α and SAT-2 in RNA polymerase II CTD repeat
  YSPTSPS (phospho S2) Recombinant Rat mAb (S-R199)-
  immunoprecipitated sample.