GAPDH Recombinant Rabbit mAb (S-240-147)

货号： S0B0261

Datasheet

价格： ￥400
规格：
25μl100μl500μl1ml
数量：

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产品规格

宿主来源

Rabbit
抗原名称

GAPDH
分子别名

Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH
免疫原

Synthetic Peptide
细胞定位

Nucleus, Membrane, Cytoplasm
Accession

P04406, P16858
克隆号

S-240-147
抗体类型

Rabbit mAb
应用

IHC-P, ICC, WB
反应种属 ?

Zf, Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Hamster (95%), Mink (95%), Pig (95%), Squirrel (95%),Dog (95%), Cat (95%), Sheep (95%), Quail (95%), Bovine (95%), Chicken (95%)
纯化方式

Protein A
浓度

1 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:10000-1:50000
IHC	1:5000
ICC	1:50-1:200

背景介绍

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme of about 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules [PMID: 17072346]. GAPDH comprises a polypeptide chain of 335 amino acids. Structural studies identified two regions, namely the glyceraldehyde-3-phosphate catalytic site and the NAD+ binding site, a primary structure known as the Rossmann fold, which is also required for the activity of other dehydrogenases. Many of these roles are dependent on the ability of GAPDH to bind different macromolecules in the cell.

免疫印迹
- WB result of GAPDH Rabbit mAb
  Primary antibody: GAPDH Rabbit mAb at 1/10000 dilution
  Lane 1: zebrafish lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 37 kDa
  Observed MW: 37 kDa
- WB result of GAPDH Rabbit mAb
  Primary antibody: GAPDH Rabbit mAb at 1/50000 dilution
  Lane 1: zebrafish lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 37 kDa
  Observed MW: 37 kDa
- WB result of GAPDH Rabbit mAb Primary antibody: GAPDH Rabbit mAb at 1/10000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: THP-1 whole cell lysate 20 µg Lane 3: MCF7 whole cell lysate 20 µg Lane 4: NIH/3T3 whole cell lysate 20 µg Lane 5: mouse spleen lysate 20 µg Lane 6: C6 whole cell lysate 20 µg Lane 7: rat spleen lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 37 kDa Observed MW: 37 kDa
- WB result of GAPDH Rabbit mAb Primary antibody: GAPDH Rabbit mAb at 1/50000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: THP-1 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 37 kDa Observed MW: 37 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human spleen. Anti-GAPDH antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti-GAPDH antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat testis. Anti-GAPDH antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-GAPDH antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti-GAPDH antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).
- ICC shows positive staining in C6 cells. Anti-GAPDH antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).