Human IgG2 F(c) Recombinant Rabbit mAb (SDT-318-75)
Human IgG2,人IgG2,人免疫球蛋白G2,Human Immunoglobulin G2,Human IgG2 Heavy Chain,Human IgG2 γ,Human IgG2γ,Human IgG2 gamma,Human IgG2 Light Chain,Human IgG2 κ,Human IgG2κ,Human IgG2 kappa,Human IgG2 λ,Human IgG2λ,Human IgG2 lambda,Human IgG2 (H+L),Human IgG2 Fab,Human IgG2 Fc,human IgG2,Hu IgG2
货号： S0B0223
- 价格： ￥600
- 规格：
- 数量：
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别名
Human IgG2,人IgG2,人免疫球蛋白G2,Human Immunoglobulin G2,Human IgG2 Heavy Chain,Human IgG2 γ,Human IgG2γ,Human IgG2 gamma,Human IgG2 Light Chain,Human IgG2 κ,Human IgG2κ,Human IgG2 kappa,Human IgG2 λ,Human IgG2λ,Human IgG2 lambda,Human IgG2 (H+L),Human IgG2 Fab,Human IgG2 Fc,human IgG2,Hu IgG2宿主来源
Rabbit抗原名称
Human IgG2免疫原
Native protein细胞定位
Cell membrane, SecretedAccession
P01859克隆号
SDT-318-75抗体类型
Recombinant mAb应用
IHC-P, ELISA, WB反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000-1:20000 |
| IHC-P | 1:2000 |
The intact antibody of human immunoglobulin (IgG) is composed of the fragment for antigen binding (Fab) and the crystallizable fragment (Fc) for binding of Fcγ receptors. Among the four subclasses of human IgG (IgG1, IgG2, IgG3, IgG4), which differ in their constant regions, particularly in their hinges and CH2 domains, IgG1 has the highest FcγR-binding affinity, followed by IgG3, IgG2, and IgG4 [PMID: 32370812].
免疫印迹
WB result of Human IgG2 Rabbit mAb
Primary antibody: Human IgG2 Rabbit mAb at 1/1000 dilution
Lane 1: IgG1 FC recombinant protein lysate 10 ng
Lane 2: IgG2 FC recombinant protein lysate 10 ng
Lane 3: IgG3 FC recombinant protein lysate 10 ng
Lane 4: IgG4 FC recombinant protein lysate 10 ng
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: IgG1 FC、IgG2 FC、IgG4 FC: 27 kDa; IgG3 FC: 33 kDa
Observed MW: IgG2 FC: 33 kDa
WB result of Human IgG2 Rabbit mAb
Primary antibody: Human IgG2 Rabbit mAb at 1/20000 dilution
Lane 1: Human serum lysate 5 μg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 52 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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