Human IgG2 F(c) Recombinant Rabbit mAb (SDT-318-75)

货号： S0B0223

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

Human IgG2
分子别名

Immunoglobulin heavy constant gamma 2, Ig gamma-2 chain C region, Ig gamma-2 chain C region DOT, Ig gamma-2 chain C region TIL, Ig gamma-2 chain C region ZIE
免疫原

Native protein
细胞定位

Cell membrane, Secreted
Accession

P01859
克隆号

SDT-318-75
抗体类型

Recombinant mAb
应用

IHC-P, ELISA, WB
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000-1:20000
IHC-P	1:2000

背景介绍

The intact antibody of human immunoglobulin (IgG) is composed of the fragment for antigen binding (Fab) and the crystallizable fragment (Fc) for binding of Fcγ receptors. Among the four subclasses of human IgG (IgG1, IgG2, IgG3, IgG4), which differ in their constant regions, particularly in their hinges and CH2 domains, IgG1 has the highest FcγR-binding affinity, followed by IgG3, IgG2, and IgG4 [PMID: 32370812].

免疫印迹
- WB result of Human IgG2 Rabbit mAb
  Primary antibody: Human IgG2 Rabbit mAb at 1/1000 dilution
  Lane 1: IgG1 FC recombinant protein lysate 10 ng
  Lane 2: IgG2 FC recombinant protein lysate 10 ng
  Lane 3: IgG3 FC recombinant protein lysate 10 ng
  Lane 4: IgG4 FC recombinant protein lysate 10 ng
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: IgG1 FC、IgG2 FC、IgG4 FC: 27 kDa; IgG3 FC: 33 kDa
  Observed MW: IgG2 FC: 33 kDa
- WB result of Human IgG2 Rabbit mAb
  Primary antibody: Human IgG2 Rabbit mAb at 1/20000 dilution
  Lane 1: Human serum lysate 5 μg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 36 kDa
  Observed MW: 52 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.