STING Recombinant Rabbit mAb (SDT-R150)

货号： S0B0084

Datasheet

价格： ￥600
规格：
25μl100 μl1ml
数量：

大包装询价

产品介绍评论(0) 引用文献(1)

产品规格

宿主来源

Rabbit
抗原名称

STING
分子别名

Hsting, ERIS, hMITA, Transmembrane protein 173
免疫原

N/A
细胞定位

Endoplasmic reticulum membrane, Cytoplasm, Mitochondrion outer membrane, Cell membrane
Accession

Q86WV6
克隆号

SDT-R150
抗体类型

Recombinant mAb
应用

IHC-P, WB, IP
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IHC-P	1:500
IP	1:50

背景介绍

Mediator of IRF3 activation (MITA, also known as stimulator of interferon genes (STING) and endoplasmic reticulum interferon stimulator (ERIS)) is an ER-associated protein that senses cellular and bacterium-derived cyclic dinucleotide (CDN), leading to induction of type-I interferons (IFNs) and innate immune responses against viruses and bacteria [PMID: 36193584].

免疫印迹
- WB result of STING Rabbit mAb Primary antibody: STING Rabbit mAb at 1/1000 dilution Lane 1: THP-1 whole cell lysate 20 µg Lane 2: HaCaT whole cell lysate 20 µg Lane 3: HT-29 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 42kDa Observed MW: 30, 37kDa
免疫沉淀
- STING Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating STING in 0.4 mg HL-60 whole cell lysate.
  Western blot was performed on the immunoprecipitate using STING Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: HL-60 whole cell lysate 20 µg (Input)
  Lane 2: STING Rabbit mAb IP in HL-60 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HL-60 whole cell lysate
  Predicted MW: 42 kDa
  Observed MW: 37 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human prostate. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.