Hemoglobin Recombinant Rabbit mAb (SDT-193-71)

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货号： S0B0057

Datasheet

价格： ￥600
规格：
25μl100μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

Hemoglobin
分子别名

Hb
免疫原

Native protein
细胞定位

Cytosol, Blood microparticle
Accession

P69905, P68871
克隆号

SDT-193-71
抗体类型

Rabbit mAb
应用

IHC-P, WB, IP
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.25 mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:500
IHC-P	1:5000
IP	1:25

背景介绍

The primary function of Hemoglobin (Hb) is to transport oxygen (O2) from the lung to tissues, binding and releasing O2 in a cooperative manner [PMID: 32189307]. Hemoglobin is a tetramer, consisting of two α-subunits (α1 and α2) and two βsubunits (β1 and β2) that are structurally similar and about the same size. The two αβ dimers (named α1β1 and α2β2) are arranged around a 2-fold axis of symmetry resulting in a large central water cavity in the T or unliganded or deoxygenated structure and a narrower cavity in the R or liganded or oxygenated structure.

免疫印迹
- WB result of Hemoglobin Rabbit mAb Primary antibody: Hemoglobin Rabbit mAb at 1/500 dilution Lane 1: 293T whole cell lysate 20 µg Lane 2: TF-1 whole cell lysate 20 µg Negative control: 293T whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 13 kDa Observed MW: 13 kDa
免疫沉淀
- Hemoglobin Rabbit mAb at 1/25 dilution (1µg) immunoprecipitating Hemoglobin in 0.4mg TF-1 whole cell lysate.
  Western blot was performed on the immunoprecipitate using Hemoglobin Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1 : TF-1 whole cell lysate 20µg(input)
  Lane 2 : Hemoglobin Rabbit mAb IP in TF-1 whole cell lysate
  Lane 3 : Rabbit monoclonal IgG IP in TF-1 whole cell lysate
  Predicted MW: 13 kDa
  Observed MW: 13 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human placenta. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human prostate. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human stomach. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.