Products Primary Antibodies Other Primary Antibodies
PG II/PGC Recombinant Rabbit mAb (SDT-194-100)
Catalog Number： S0B0048
- Price： $52
- Size：
- Quantity：
Bulk Quotation 
Product Introduction comment(0) Citations()
Host
RabbitAntigen
PG II/PGCSynonyms
Gastricsin, Pepsinogen CImmunogen
Recombinant ProteinLocation
SecretedAccession
P20142Clone Number
SDT-194-100Antibody Type
Rabbit mAbApplication
IHC-P, WBReactivity ?
Hu, Ms, RtPurification
Protein AConcentration
0.25 mg/mlPhysical Appearance
LiquidStorage Buffer
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300Stability & Storage
12 months from date of receipt / reconstitution, -20 °C as supplied
| application | dilution |
|---|---|
| IHC-P | 1:1000 |
| WB | 1:2500 |
- Pepsinogen (PG) is the precursor of pepsin and is composed of five hypotypes: pepsinogen A (PGA), pepsinogen B (PGB), pepsinogen C (PGC), pepsinogen F (PGF) and chymosinogen [PMID: 28546787]. Pepsinogen C, also known as progastricsin or PG II, is an aspartic protease expressed primarily in gastric chief cells [PMID: 14578117].
Western Blot
![item.picture[0].fileOrgName](https://univ-sdt.oss-cn-shanghai.aliyuncs.com/prod/202305/9fe1e8be0cca4e3384036a792192feaa.jpg)
WB result of PG II/PGC Rabbit mAb
Primary antibody: PG II/PGC Rabbit mAb at 1/2500 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse stomach lysate 20 µg
Negative control: mouse liver lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 38 kDa
Immunohistochemistry
![item.picture[0].fileOrgName](https://univ-sdt.oss-cn-shanghai.aliyuncs.com/prod/202305/acb639e993bd44a9897672d01a0c66ad.jpg)
IHC shows positive staining in paraffin-embedded human stomach. Anti-PG II/PGC antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
![item.picture[0].fileOrgName](https://univ-sdt.oss-cn-shanghai.aliyuncs.com/prod/202305/d9d325e8a5194881b6a455a71cde871c.jpg)
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-PG II/PGC antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
![item.picture[0].fileOrgName](https://univ-sdt.oss-cn-shanghai.aliyuncs.com/prod/202305/eb15a947b25744b3bc0b8d638dde864a.jpg)
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-PG II/PGC antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
![item.picture[0].fileOrgName](https://univ-sdt.oss-cn-shanghai.aliyuncs.com/prod/202305/3acbe24c658141cba6be63d8395e0e4b.jpg)
Negative control: IHC shows negative staining in paraffin-embedded mouse liver. Anti-PG II/PGC antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
![item.picture[0].fileOrgName](https://univ-sdt.oss-cn-shanghai.aliyuncs.com/prod/202305/d8031c29c55c43ab83f2da37b8bc59d6.jpg)
IHC shows positive staining in paraffin-embedded rat stomach. Anti-PG II/PGC antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Citations()
comment(0)