β-actin Rabbit mAb (SDT-R015)

货号： S0B0005

Datasheet

价格： ￥400
规格：
25μl100μl500ul1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

β-Actin
分子别名

ACTB
免疫原

Recombinant Protein
细胞定位

Cytoplasm, Cytoskeleton
Accession

P60709
克隆号

SDT-R015
抗体类型

Rabbit mAb
应用

IHC-P, ICC, WB, FC(Intra)
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5mg/ml
分子量

42kDa
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
ICC	1:500
WB	1:1000-1:5000
FC(Intra)	1:500
IHC-P	1:2000

背景介绍

Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.

免疫印迹
- WB result of β-actin Rabbit mAb
  Primary antibody: β-actin Rabbit mAb at 1/1000 dilution
  Lane 1: Hela whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 42 kDa
  Observed MW: 42 kDa
- WB result of β-actin Rabbit mAb
  Primary antibody: β-actin Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 lysate 20 µg
  Lane 2: mouse kidney lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 42 kDa
  Observed MW: 42 kDa
- WB result of β-actin Rabbit mAb
  Primary antibody: β-actin Rabbit mAb at 1/1000 dilution
  Lane 1: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 42 kDa
  Observed MW: 42 kDa
- WB result of β-actin Rabbit mAb
  Primary antibody: β-actin Rabbit mAb at 1/5000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: NIH/3T3 whole cell lysate 20 µg
  Lane 3: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 42 kDa
  Observed MW: 42 kDa
流式分析
- Flow cytometric analysis of HeLa cells labelling β-actin antibody at 1/500 (0.1ug) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
- IHC shows positive staining in paraffin-embedded smooth msucle of human skeletal muscle.
  Anti-β-actin antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon.
  Anti-β-actin antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer.
  Anti-β-actin antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung cancer.
  Anti-β-actin antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse liver.
  Anti-β-actin antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex.
  Anti-β-actin antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows cytoplasm staining in HeLa cells.
  Anti-β-actin antibody was used at 1/500 dilution and incubated overnight at 4°C.
  Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution.
  The cells were fixed with 100% Methanol and permeabilized with 0.1% PBS-Triton X-100.
  Nuclei were counterstained with DAPI.
- ICC shows cytoplasm staining in NIH3T3 cells.
  Anti-β-actin antibody was used at 1/500 dilution and incubated overnight at 4°C.
  Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution.
  The cells were fixed with 100% Methanol and permeabilized with 0.1% PBS-Triton X-100.
  Nuclei were counterstained with DAPI.