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产品中心 诊断抗原 病毒蛋白

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SARS-CoV-2 RBD, His Tag

价格 ￥1,800.00 （ 供应商现货 ： 3-5个工作日）
货号 S0A2008
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    SARS-CoV-2

  • Accession

    P0DTC2

  • 表达序列

    RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCY GVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNN LDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPT NGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKGGGGSHHHHHHHHHH.

  • 表达宿主

    HEK293

  • 分子量

    26.3 kDa (reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 内毒素含量

    <1EU/μg

  • 标记

    Unconjugated

  • 标签

    with C-His Tag

  • 性状

    Lyophilized powder

  • 缓冲体系

    Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4 with 3% trehalose.

  • 溶解方法

    Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.

  • 储存条件

    12 months from date of receipt, -20 to -70 °C as supplied.
    6 months, -20 to -70 °C under sterile conditions after reconstitution.
    1 week, 2 to 8 °C under sterile conditions after reconstitution.
    Please avoid repeated freeze-thaw cycles.

背景介绍

  • Sars-cov-2 (2019-NCOV) infects human respiratory epithelial cells through binding to human ACE2 receptors. Spike protein is a large type I transmembrane protein containing two subunits S1 and S2.S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing cell surface receptors. S2 contains the basic elements required for membrane fusion. S protein plays a key role in inducing neutralizing antibody and T cell responses as well as protective immunity. This product is the recombinant human SARS-CoV-2 RBD protein expressed from human 293 cells (HEK293).

  • 生物活性

    • Immobilized SARS-CoV-2 RBD, His Tag at 4 μg/mL (50 μL/well) can bind Human ACE2, hFc tag (S0A0071) with EC50 of 4.121-5.034 ng/ml.

  • 电泳

    • 1μg（R: reducing conditions）

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