SARS-CoV-2 RBD, His Tag

Catalog Number： S0A2008

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Product Specification

Species

SARS-CoV-2
Accession

P0DTC2
Amino Acid Sequence

RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCY GVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNN LDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPT NGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKGGGGSHHHHHHHHHH.
Expression System

HEK293
Molecular Weight

26.3 kDa (reducing)
Purity

＞95% by SDS-PAGE
Endotoxin

<1EU/μg
Conjugation

Unconjugated
Physical Appearance

Lyophilized powder
Storage Buffer

0.2M PBS, pH7.4
Reconstitution

Reconstitute at less than 1 mg/mL according to the size in deionized water after rapid centrifugation.
Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Background

Sars-cov-2 (2019-NCOV) infects human respiratory epithelial cells through binding to human ACE2 receptors. Spike protein is a large type I transmembrane protein containing two subunits S1 and S2.S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing cell surface receptors. S2 contains the basic elements required for membrane fusion. S protein plays a key role in inducing neutralizing antibody and T cell responses as well as protective immunity. This product is the recombinant human SARS-CoV-2 RBD protein expressed from human 293 cells (HEK293).

Bioactivity
- Immobilized SARS-CoV-2 RBD, His Tag at 4 μg/mL (50 μL/well) can bind Human ACE2, hFc tag (S0A0071) with EC50 of 4.121-5.034 ng/ml.
SDS-PAGE
- 1μg（R: reducing conditions）